Abstract:To overcome the issue of multidrug resistant (MDR) microbes, the exploration of ways to improve the antimicrobial efficiency of existing antibiotics is one of the promising approaches. In search of synthons with higher efficiency, in current investigations, cocrystal and amorphous salt of levofloxacin hemihydrate (LEV) were developed with phthalimide (PTH) and caffeic acid (CFA). New materials were characterized with the help of FT-IR, Raman spectroscopy, powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Shifting, attenuation, appearance/disappearance and broadening of bands were observed in the FT-IR and Raman spectra of the materials as evidence of the required product. The PXRD diffraction pattern observed for LEV-PTH indicated cocrystal while halo diffractogram of LEV-CFA revealed amorphous nature. DSC/TG analysis confirmed the hydrated nature of the cocrystal/salt. The dissolution rate and antimicrobial activity against selected strains, K. pneumonia, E. coli and S. typhi of parent drug and the new material were compared. The zone of inhibition (ZI) observed for 5 µg LEV-PTH was 30.4 + 0.36 (K. pneumonia), 26.33 + 0.35 (E. coli) and 30.03 + 0.25 mm (S. typhi) while LEV-CFA salt (5 µg) against the same strains inhibited 33.96 ± 0.25, 31.66 ± 0.35 and 27.93 ± 0.40 mm, respectively. These novel formulations enhance the dissolution rate as well as antibacterial efficiency and are expected to be potent against MDR bacterial strains.Keywords: levofloxacin/phthalimide; levofloxacin/caffeic acid; heterosynthons; antibacterial activity; MDR strains
Meropenem injection is used to treat skin and abdominal (stomach area) infections caused by bacteria and meningitis (infection of the membranes that surround the brain and spinal cord) in adults and children 3 months of age and older. Meropenem injection is in a class of medications called antibiotics. It works by killing bacteria that cause infection.
Antibiotics such as meropenem injection will not work for colds, flu, or other viral infections. Taking antibiotics when they are not needed increases your risk of getting an infection later that resists antibiotic treatment.
Use meropenem injection until you finish the prescription, even if you feel better. If you stop using meropenem injection too soon or if you skip doses, your infection may not be completely treated and the bacteria may become resistant to antibiotics.
The compelling story of Mallory Smith, who lived with cystic fibrosis for 25 years and died in 2017 after a 10-year battle with antibiotic-resistant infections, set the stage for a discussion on Capitol Hill on May 18 about the growing impact of resistance, the broken economic model for antibiotic development, the need to use antibiotics wisely and how legislation like the PASTEUR Act can help.
Sharing her perspective as an ID physician who focuses on the appropriate use of antibiotics, panelist Sara Cosgrove, MD, MS, FIDSA, highlighted the example of colistin, a decades-old antibiotic previously discontinued because of its toxicity that since has been brought back into clinical use, out of desperation, to treat increasingly resistant infections.
The PASTEUR Act, championed by IDSA, would help revitalize antibiotic research and development. The bipartisan legislation would establish a subscription-style system in which the federal government pays companies a set fee to access their novel antibiotics, regardless of how much of the drug is used.
This approach would give companies a predictable return on their investments in antibiotic research, providing the income necessary to avoid bankruptcy and continue manufacturing their antibiotic while attracting more private investment to support additional research. PASTEUR would also provide more funds for antibiotic stewardship programs at hospitals, focusing on those with the greatest need, to help safeguard the effectiveness of new antibiotics and slow the evolution of superbugs.
Previous studies have implicated the novel peptide antibiotic human beta-defensin 1 (hBD-1) in the pathogenesis of cystic fibrosis. We describe in this report the isolation and characterization of the second member of this defensin family, human beta-defensin 2 (hBD-2). A cDNA for hBD-2 was identified by homology to hBD-1. hBD-2 is expressed diffusely throughout epithelia of many organs, including the lung, where it is found in the surface epithelia and serous cells of the submucosal glands. A specific antibody made of recombinant peptide detected hBD-2 in airway surface fluid of human lung. The fully processed peptide has broad antibacterial activity against many organisms, which is salt sensitive and synergistic with lysozyme and lactoferrin. These data suggest the existence of a family of beta-defensin molecules on mucosal surfaces that in the aggregate contributes to normal host defense.
The researchers also tested the effect of an extremely high-salt diet in mice with persistent footpad infections. Salt stores at the site of the infection increased after consumption of the high-salt diet, and the infections cleared up.
Toward that end, Titze and his colleagues have received support from a Strategically Focused Prevention Research Network Centers grant from the American Heart Association to find out whether salt stores can be mobilized by dietary lifestyle changes or drugs.
Vaccine ingredients can look unfamiliar. However, it is important to remember that many of the substances used in vaccines are found naturally in the body. For example, many vaccines contain salts based on sodium and potassium (see the section on 'Acidity regulators'), which are essential for life. People may think of formaldehyde as a man-made chemical, but in small quantities it is also found naturally in the bloodstream.
All vaccine ingredients are present in very small quantities, and there is no evidence that they cause harm in these amounts. The exception to this is the small number of people who may be severely allergic to a vaccine ingredient, even if it is present only in trace amounts (for example, egg proteins or antibiotics used in vaccine manufacture). If you look up some vaccine ingredients on the internet you may read that they could be harmful, but most of them are present in vaccines in amounts that are completely normal for our bodies. Even common salt (sodium chloride), which is essential for normal functioning of the body, is harmful in large quantities.
Many vaccines contain aluminium salts such as aluminium hydroxide, aluminium phosphate or potassium aluminium sulphate. They act as adjuvants, strengthening and lengthening the immune response to the vaccine. Aluminium salts appear to slow down the release of the active ingredient from the vaccine once it is injected, and stimulate the immune system to respond to the vaccine. They also absorb protein well, and stop the proteins in the vaccine sticking to the walls of a container during storage.
The amount of aluminium present in vaccines is small - less than 2 milligrams of the salts, and less than a milligram of actual aluminium. In the UK, the highest dose of aluminium that babies receive in one go from vaccines is just under 1.5 milligrams (from the 6-in-1, PCV and MenB vaccines at 8 weeks and 16 weeks). A study from 2011 modelled the impact of aluminium from diet and vaccines in infants, and concluded that the total amount of aluminium absorbed from both sources was likely to be less than the weekly safe intake level. A study from 2002 drew similar conclusions. A study published in March 2018 took samples of blood and hair from 85 babies and measured their levels of aluminium. These levels varied considerably, but researchers did not find any correlation between aluminium levels in blood or hair and the estimated amount of aluminium that the babies had received from vaccines.
Antibiotics are used during the manufacturing process of some vaccines to stop bacteria growing and contaminating the vaccine. However, antibiotics which commonly cause allergic reactions (such as penicillins, cephalosporins and sulphonamides) are not used in vaccines. Traces of five antibiotics may be found in some of the vaccines used in the UK. These are neomycin, streptomycin, polymyxin b, gentamicin and kanamycin. People with a known allergy to any of these antibiotics should ask for expert advice before receiving these vaccines.
If the nipple is obviously infected, then talk to your health care provider about the possibility of using an oral (systemic) antibiotic. One study indicated that topical antibiotics and good breastfeeding techniques might not be sufficient if infection is present. (See Livingstone V, Stringer LJ. The treatment of Staphyloccocus aureus infected sore nipples: a randomized comparative study. J Hum Lact. 1999 Sep;15(3):241-6.) See also Oral antibiotic use for sore, cracked nipples and Staphylococcus aureus and sore nipples.)Additional informationTreatment of Sore, Cracked, or Bleeding Nipples by Becky Flora, IBCLC
Hi all, I'm doing some susceptibility testing and am purchasing antibiotics. I had a question about whether to use the salt form or the pure form, for example for piperacillin: -205808-piperacillin-sodium.html -205807-piperacillin.html I would think the pure form hydrolyzes in solution anyway. But in what situations would one be better than the other? Thanks! 2b1af7f3a8