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Adobe Acrobat X Pro Serial Number Full Version Free Download. 25 Feb 2016 · Full Version Download Free Download Adobe Acrobat X Pro Serial Number. 19 Feb 2016 · .Isolation, and Partial Characterization, of a Phytase from a Nitrite-Tolerant Bacillus sp. and Its Viability under High Concentration of Nitrite.
A psychrotolerant Bacillus strain EK-2 was isolated from a rural chicken feed lot. The strain was tolerant to various concentrations of nitrite, including 2500 mg kg(-1) NO(2)(-) and 5000 mg kg(-1) NO(2)(-), and produced high phytase activity. Isolation of a phytase from this bacterium with potential activity under high concentrations of nitrite is reported in the present study. After application of a heat treatment for 30 min at 100 °C, the strain produced one phytase with an enzyme activity of 65 U mg(-1) protein and one with an enzyme activity of 100 U mg(-1) protein, when measured at pH 5.0 at 55 °C. The purified enzymes were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, with molecular weights of approximately 42 and 48 kDa, respectively. The enzyme activity was inhibited by EDTA, Fe(2+) ions, and Cu(2+) ions. The NH(4)(+) ions had no significant effect on the enzyme activity. However, the enzyme activity was increased when measured under the presence of Na(+) and K(+) ions. The optimum temperature for the enzyme activity was 35 °C, and the half-life of the enzyme at 50 °C was estimated to be 2.5 h. The enzyme had a half-life of 50 min at 60 °C and lost 72% of its activity when measured in the presence of 2500 mg kg(-1) NO(2)(-). The enzyme exhibited significant activity in the presence of 500 mg kg(-1) Ca(2+) at pH 8.5, which corresponded to a possible role of the enzyme in overcoming calcium phosphate hydrolysis in animal feed, due 0b46394aab